Mitchell Dushay
Assistant Professor of Biology
Office: 204 Life Sciences
Office Hours:
Phone: 312-567-8849
Fax: 312-567-3494
Email:
mdushay@iit.edu
Web:
Expertise
Education
- B.A, Brown University
- Ph.D, Brandeis University
Curriculum Vitae
Research & Major Accomplishments
Insects and the majority of the world's animal species lack antibodies and T cells (adaptive immunity), yet they still depend on immune defenses against infection to be able to survive and reproduce. The fruit fly, Drosophila melanogaster, is a powerful model for the study of insect immunity. Besides insects, Drosophila research has provided insight into innate immunity in mammals including human, extending to the initial recognition of nonself (infection). Coagulation serves immune as well as physiological roles, but it is still poorly understood in insects. In collaboration with Dr U. Theopold, Stockholm University, we have identified proteins in the larval clot, and the next steps are to learn how these proteins interact to form a clot, how coagulation is triggered and regulated, how clots contribute to wound closure and repair, and how they protect against infection.
Another project concerns lamins; intermediate filament proteins that form a matrix underlying the nuclear membrane. Lamins contribute to nuclear strength and shape, participate in cell division, and affect cell type-specific gene expression. Mutations in a human lamin gene cause a wide range of diseases, including muscular dystrophies and accelerated aging disorders such as Hutchinson-Guilford Progeria Syndrome that causes death by age 14 of atherosclerosis, heart attack, and stroke. Mutations in the Drosophila lamin (lam) gene cause early death. The few lam adults that emerge survive only a few days and are weak, uncoordinated, and flightless – they behave like old flies. Mutant larvae also move poorly and show immune defects. We are using molecular, bioinformatic, and functional genomic approaches to study what other genes are involved and how lam mutations cause these phenotypes. In addition to immunity, these experiments have implications for muscular dystrophy and aging.
Current Projects
Awards/Honors
Patents
Books
Selected Publications
Lindgren, M., R. Riazi, C. Lesch, C. Wilehlmsson, U. Theopold, and M.S. Dushay (2008). "Fondue and Transglutaminase in the Drosophila larval clot." Journal of Insect Physiology 54: 586 – 592.
Muñoz-Alarcón, A., M.Pavlovic, J.Wismar, B. Schmitt, M.Eriksson, P.Kylsten, and M.S.Dushay (2007). " Characterization of lamin mutation phenotypes in Drosophila and comparison to human laminopathies." PLoS ONE Doi 10.1371/journal.pone.0000532
Lesch, C., A.Goto, M.Lindgren, G.Bidla, M.S.Dushay, and U.Theopold (2007). “Hemolectin in coag-ulation and immunity in Drosophila.” Developmental and Comparative Immunology 31: 1255-63.
Bidla, G., M.S.Dushay, and U.Theopold (2007). "Crystal cell rupture after injury in Drosophila requires JNK, small GTPases, and the TNF homolog Eiger." J. Cell Science 120: 1209 - 1215.
Agianian, B., C. Lesch, O. Loseva, and M.S.Dushay (2007). "Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae." Developmental and Comparative Immunology 31: 879 - 888.
Korayem, A.M, T.Hauling, C.Lesch, M.Fabbri, M.Lindgren, O.Loseva, O.Schmidt, M.S.Dushay,and U.Theopold (2007). "Immune function of lepidopteran silk proteins." Biochemical and Biophysical Research Communications 352: 317-22.
Theopold, U., and M.S. Dushay (2007). "Mechanisms of Drosophila Immunity—An Immune System at Work." Current Immunology Reviews 3: 276 – 288.
Scherfer, C., M.R.Qazi, K. Takahaski, R.Ueda, M.S.Dushay, U.Theopold, and B.Lemaitre (2006). "The Toll immune-regulated Drosophila protein Fondue is involved in hemolymph clotting and puparium formation." Developmental Biology 295: 156 - 163.
Bidla, G., M.Lindgren, U.Theopold, and M.S.Dushay (2005). "Hemolymph Coagulation and phenol-oxidase in Drosophila Larvae." Developmental and Comparative Immunology 29: 669 - 679.
Karlsson, C., A.M.Korayem, C.Scherfer, O.Loseva, M.S.Dushay, and U.Theopold (2004). "Prote-omic analysis of the Drosophila larval hemolymph clot." J. Biological Chemistry 279: 52033 – 41.
Korayem, A.M., M.Fabbri, K.Takahashi, C.Scherfer, M.Lindgren, O.Schmidt, R.Ueda, M.S.Dushay, and U.Theopold (2004). "A Drosophila salivary gland mucin is also expressed in immune tissues: evidence for a function in hemolymph coagulation." Insect Biochemistry and Molecular Biology 34: 1297 - 1304.
Scherfer, C., C.Karlsson, O.Loseva, G.Bidla, A.Goto, J.Havemann, M.S.Dushay, and U.Theopold (2004). "Isolation and characterization of hemolymph clotting factors in Drosophila melanogaster by a pull-out method." Current Biology 14: 625 - 629.
