John J. Kilbane , Ph.D.Research Professor of Biology
- Project Management, Biofuels R&D, Gene Expression in Diverse Hosts (aerobic, anaerobic, and thermophilic bacterial species), Biocatalysts Development, Metabolic Engineering, Strain Improvement, Mutagenesis, Fermentation, Process Optimization, Method Development, Bioremediation, Sales of Scientific R&D, Market Analysis, Strategic Planning, and Proposal Writing
- B.S. The Ohio State University
- Ph.D. Tufts University
Research & Major Accomplishments
My research focuses on applying biotechnology to solve various problems in the energy industry. Research topics include the isolation and characterization of microbial cultures with unique biochemical abilities, biodesulfurization of petroleum, using genetic engineering and metabolic engineering to develop improved biocatalysts, bioconversion of organic material into methane, ethanol or biodiesel, genetic expression studies in Rhodococcus, Thermus, and methanotrophs, development of genetic tests to detect and quantify microorganisms associated with corrosion, development of novel techniques to characterize and access microbial biodiversity, and the bioremediation of contaminated soil and water.
Previous positions included developing biocatalysts to convert lignocellulosic material to ethanol for biocatalysis, biofuels production, environmental remediation, and other topics.
- Biodesulfurization of Heavy Crude Oil
- 5,002,889 (1991) "Mutant Microorganisms Useful for Cleavage of Organic C-S Bonds", J.J. Kilbane.
- 5,104,801 (1992) "Mutant Microorganisms Useful for Cleavage of Organic C-S Bonds", J.J. Kilbane.
- 5,132,219 (1992) "Enzymes from Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969 and Mixtures Thereof for Cleavage of Organic C-S Bonds of Carbonaceous Material", J.J. Kilbane.
- 5,198,341 (1993) "Useful for Cleavage of Organic C-S Bonds Bacillus sphaeicus Microorganism", J.J. Kilbane.
- 5,344,778 (1994) "Process for Enzymatic Cleavage of C-S Bonds and Process for Reducing the Sulfur Content of Sulfur-Containing Organic Carbonaceous Material", J.J. Kilbane,
- 5,358,869 (1994) "Microbial Cleavage of Organic C-S Bonds", J.J. Kilbane.
- 5,358,870 (1994) "Microemulsion Process for Direct Biocatalytic Desulfurization of Organosulfur Molecules", D.J. Monticello and J.J. Kilbane.
- 5,516,677 (1996) "Enzyme from Rhodococcus rhodochrous ATCC 53968, Bacillus shpaericus ATCC 53969 or a Mutant Thereof for Cleavage of Organic C-S Bonds", J.J. Kilbane.
- 5,610,065 (1997) "Integrated Chemical/Biological Treatment of Organic Waste", R.L. Kelley, V.J. Srivastava and J.J. Kilbane.
- 5,750,065 (1998) "Adsorption of PCB's Using Biosorbents", J.J. Kilbane.
- 6,204,048 (2001) "Microorganisms Useful for Cleavage of Organic C-N bonds", J.J. Kilbane II, C.M.S. Ribeiro, M.M. Linhares.
- 6,221,651 (2001) "Psuedomonas ayucida Useful for Cleavage of Organic C-N Bonds", J.J. Kilbane II, C.M.S. Ribeiro, M.M. Linhares.
- 6,541,240 B1 (2003) "Bacterial Cleavage of Only Organic C-N Bonds of Carbonaceous Materials to Reduce Nitrogen Content", J.J. Kilbane II, C.M.S. Ribeiro, and M.M. Linhares.
- 6,861,258 (2005) "System and Method for Introduction and Stabilization of Genes in Thermus sp." K.J. Kayser, H.S. Park, and J.J. Kilbane II.
- 6,943,006 (2005) "Method for Metabolizing Carbazole in Petroleum", K.J. Kayser and J.J. Kilbane II.
Selected Publications“Characterization of the dszABC
Genes of Gordonia amicalis F.5.25.8 and Identification of Conserved Protein and DNA Sequences”, Kilbane II, J. J., and J. Robbins. Applied Microbiology & Biotechnology 75: 843-851 (2007).
“Microbial Biocatalysts to Upgrade Fossil Fuels”, Kilbane II, J. J. Current Opinion in Biotechnology 17: 305-314 (2006).
“Rapid Detection and High-Resolution Discrimination of the Genus Streptomyces based on 16S-23S rDNA Spacer Region and Denaturing Gradient Gel Electrophoresis” H.-S. Park, and J. J. Kilbane II. Journal of Industrial Microbiology & Biotechnology 33: 289-297 (2006).
“New Method to Characterize Microbial Diversity Using Flow Cytometry.” H.-S. Park, R. Schumacher, and J. J. Kilbane II. Journal of Industrial Microbiology & Biotechnology 32: 94-102 (2005).
“Quantifying the Contribution of Various Bacterial Groups to Microbiologically Influenced Corrosion,” Kilbane II, J. J., B. Bogan, and B. Lamb, Corrosion2005, Paper 05491, NACE International, Houston, TX pp. 1-9 (2005).
“Faster and More Accurate Data Collection for Microbiologically Influenced Corrosion,” Zhu, X. Y., and J. J. Kilbane II, Society of Petroleum Engineers SPE-93089-PP, pp. 1-9 (2005).
“Gene Expression Studies of Thermus thermophilus promoters PdnaK, Parg, and Pscs-mdh”, H.-S. Park, and J. J. Kilbane II. Lett. Appl. Microbiol. 38: 415-422 (2004).
“Heterologous Gene Expression in Thermus thermophilus:β-Galactosidase, Dibenzothiophene monooxygenase, PNB Carboxy Esterase, 2-Aminobiphenyl-2,3-diol Dioxygenase, and Chloramphenicol Acetyl Transferase.” H.-S. Park, K. J. Kayser, J.-H. Kwak, and J. J. Kilbane II. Journal of Industrial Microbiology & Biotechnology 31: 189-197 (2004).
“Development of an Environmentally Benign Microbial Inhibitor to Control Internal Pipeline Corrosion,” Bogan, B., B. M. Lamb, G. Husmillo, K. Lowe, J. R. Paterek, and J. J. Kilbane II, Final Report, DOE project DE-FC26-01NT41158, December (2004).
“Improved Method for Monitoring Microbial Communities in Gas Pipelines,” X. Zhu, J. Lubeck, K. Lowe, A. Daram, and J. J. Kilbane II, Corrosion2004 Paper No. 04592, NACE Houston, TX, pp. 1-13 (2004).
“Petroleum Biorefining: The Selective Removal of Sulfur, Nitrogen, and Metals,” J. J. Kilbane II, and S. Le Borgne, In: Petroleum Biotechnology, Studies in Surface Science and Catalysis, Volume 151 (R. Vazquez-Duhalt and R. Quintero-Ramirez, editors) pp. 29-65 (2004).
“Biotechnological Upgrading of Fossil Fuel,” J. J. Kilbane II. In: Petroleum Microbiology (B. Ollivier and M. Magot, editors) ASM Press, Washington D.C. pp. 239-256 (2004).
“Characterization of Microbial Communities in Gas Industry Pipelines,” Zhu, Y. X., J. Lubeck, and J. J. Kilbane II, Appl. Environ. Microbiol. 69: 5354-5363 (2003).
“Ethanol-Based Foam Stability as Probed by Foam Lamella Thinning,” Sethumadhavan, G. N., A. D. Nikolov, D. A. Wasan, V. J. Srivastava, J. J. Kilbane II, and T. D. Hayes, Indust. Eng. Chem. Res. 42:2634-2638 (2003).
“Applications of Biotechnolgy to the Energy Industry”, Kilbane II, J. J. Oil Asia Journal, pp. 19-25, May (2003).
“Effect of Aeration and Agitation on the Growth rate of Thermus thermophilus in Batch Mode,” M. U. Demirtas, A. Kolhatkar, and J. J. Kilbane II, J. Biotechnology & Bioengineering 95: 113-117 (2003).