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While interactive tutorials have existed at the grammar and high school levels for some time now, there is still little material on the market at the college level, especially in the area of Physics and Engineering. The aim of this project is to develop interactive tutorials for students in college level courses through the use of Hypercard. Hypercard allows for the easy integration of multimedia into tutorials, which is often a necessity in getting the concepts in advanced material across to students. A base template will be developed so that instructors in any class can create custom tutorials to serve the needs of their students. These tutorials will allow students to view topics an many levels as well as to cross reference between topics. The hope is to develop a self-paced learning experience that will serve students at all academic levels.
Detailed genetic maps are the key to understanding variability in Ustilago horder and U. violacea. We have reported the use of auxotrophic, drug resistant and morphological markers in development of linkage map of U.horder. This study investigated the production of restriction fragments from U. hordei and U. violacea for use as markers. The cells were Iyophilized, pulverized, and DNA extracted by first adding Iysis buffer and 10% CTAB in 0.7M NaCI, followed by phenol/chloroform/isoamyl alcohol. The mixture was incubated for one hour at 65C and centrifuged. RNase Awas added to the supernatant and the mixture incubated at 37C for 15 min. followed by the addition of chloroform. The sample was centrifuged and the supernatant treated to remove polysaccharides by the ethanol precipitation method. DNA was precipitated from the supernatant with isopropanol, washed with 70% ethanol, dried and dissolved in TE buffer. DNA was digested with the restriction enzymes BamHI, EcoRl, Pstl and Hindlll. The samples were electrophoresed on a 1% agarose gel. The result indicated differences in banding patterns. This technique will be useful in the detection of RFLPs which will be employed to construct genomic libraries for hybridization studies. (Suported by Grant NIH GM 08043)
ICN is an educational lesson retrieval system or digital library which allows the educational community to obtain and make comments about the lessons that are currently in the database. At this time, ICN consists of lesson plans in the areas of math and science.
First, I will present a brief Internet tour. Then, I will describe the system's interface, present and future implementations. Finally, I will present a brief tour of the Eiffel programming language that will be used in the development of the future ICN.
Spirulina Maxima is a blue-green microalgae that grows naturally on Lake Texcoco in Mexico and Lake Chad in Africa. It is also grown in artificial ponds. Spirulina is used as a whole food which is high in protein and overall nutrition, making it a viable solution to global malnutrition. This research tests the effects of sewage effluent and metal contaminants on the algae culture growth rate. This is pertinent to wastewater treatment in many regions of the world, because algae treated sewage is comparitively cheaper and also improves sanitation.
The Spirulina cultures were put in complete nutrient solution optimal for growth in the laboratory under flourescent lamps. Cultures were also placed outdoors under natural climatic conditions. Different concentrations of lead were added to the separate cultures. Cadmium was also added to the laboratory experiment to compare its toxicity to that of lead on Spirulina. Temperature, humidity, and light intensity were monitored throughout the experiment to know the condition of growth. By measuring algal density and chlorophyll levels, it was concluded that the metals had a deleterious effect on Spirulina's growth rate proportional to the amount and concentration of the metal
The purpose of the study was to determine whether perinatal exposure to cocaine alters normal sexual development in rats. The significance of the study is that it will allow us todetermine if exposure to cocaine causes low birth weight in the offspring. Cocaine hydrochloride in saline was administered to pregnant female rats via subcutaneous injection (0, 7.5, 15, or 30 mg/kg body weight). The litters were pulled to five males and five females on the day of birth. The pups were injected with cocaine or saline corresponding to their mother's treatment group. The litters' reproductive cyclicity was monitored via vaginal ravage in females. The males in the litter were handled daily to avoid any artifactual differences between males and females. On day 60 the offspring were gonadectomized. Steroids were injected subcutaneously to induce LH surges and blood samples were later taken to measure the surge. Completion of this experiment will allow us to determine the effect cocaine has on sexual development and reproductive cyclicity in rats.
Conventional ceramic techniques for synthesizing single phase cuprate superconductors typically involve multiple high-temperature (800C) firings with intermediate mixing and grinding. This process yields particle sizes of several microns to tens of microns.
Such large particle sizes have been implicated in grain connectivity problems, particularly in wire fabrication. Low temperature (600C) synthesis from highly reactive metal hydroxide precursors solve this problem in two ways: First, the precursors powders have extremely fine (submicron) particle size, and, second, the low temperature of the reactions minimizes loss of materials and ensures homogeneity in the sample matrix. We describe synthetic methods for forming high quality powders of T12Ba2CuO6(T1-2201), T12Ba2CaCu208 (T1-2212), Bi2Sr2CaCu202 (Bi-2212), YBa2Cu307-Y- 123) at low temperatures and/or extremely short reaction times (10 fur). The effect of nominal composition, temperature, and time on synthesis and superconducting transition temperature are discussed. Presently, superconductivity has not been found in Y-123 with this method but research in this area is on going.