PROBES AND PRIMER SEQUENCES SPECIFIC TO CLOSTRIDIUM BOTULINUM TYPE A TOXIN
The figure below shows a schematic diagram of the relative locations of some hybridization probes and PCR primer sets specific to the C. botulinum Type A Toxin gene. Information about each probe or primer set is given below. This list is not intended to be comprehensive, but to serve as a starting point.
The sequence of the C. botulinum Type A toxin gene can be found at GenBank accession M30196.
Hybridization Probe Sequences
| Probe # | Sequence (5'-3') | Reference |
| CBT05 | attagataaaggatacaataaggc |
PCR Primer Pair Sequences
| Primer Pair # | Primer A (5'-3') | Primer B (5'-3') | Reference |
| agttgctatgtgtaagaggg | gaacggttagaaccttattcgc | ||
| gatcctgtaaatggtgttg | caagtcccaattattaactttgat | ||
| tatggaatagcaattaatcc | gtgtaatttaccttaggtac |
References
1. Fach, P., J. Guillou, and M. Popoff. 1993. Polymerase Chain Reaction for the Rapid Detection of Clostridium botulinum Type A Strains and Detection in Food Samples. J. Applied Bacteriology 75; 234-239.
2. Ferreira, J., M. Hamdy, S. McCay, and B. Baumstark. 1992. An Improved Assay for Identification of Type A Clostridium botulinum Using the Polymerase Chain Reaction. J. Rapid Methods and Automation in Microbiology 1; 29-39.
3. Szabo, E., J. Pemberton, and P. Desmarcherlier. 1993. Detection of the Genes Encoding Botulinum Neurotoxin Types A to E by the Polymerase Chain Reaction. App. Env. Microbiol. 59; 3011-3020.
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