PROBES AND PRIMER SEQUENCES SPECIFIC TO CLOSTRIDIUM BOTULINUM TYPE E TOXIN
The figure below shows a schematic diagram of the relative locations of some hybridization probes and PCR primer sets specific to the C. botulinum Type EToxin gene. Information about each probe or primer set is given below. This list is not intended to be comprehensive, but to serve as a starting point.
The sequence of the C. botulinum Type E toxin gene can be found at GenBank accession X62683.
Hybridization Probe Sequences
| Probe # | Sequence (5'-3') | Reference |
| CBT06 | gagcttacaaataaatatgatattaagc |
PCR Primer Sequences
| Primer Pair # | Primer A (5'-3') | Primer B(5'-3') | Reference |
| tatatattaaaccaggcgg | tagagaaatattggaactg |
References
1. Campbell, K., M. Collins, and A. East. 1993. Gene Probes for Identification of the Botulinal Neurotoxin Gene and Specific Identification of Neurotoxin Types B, E, and F. J. Clin. Microbiol. 31; 2255-2262.
2. Szabo, E., J. Pemberton, and P. Desmarcherlier. 1993. Detection of the Genes Encoding Botulinum Neurotoxin Types A to E by the Polymerase Chain Reaction. App. Env. Microbiol. 59; 3011-3020.
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