PROBES AND PRIMER SEQUENCES SPECIFIC TO CLOSTRIDIUM BOTULINUM TYPE F TOXIN
The figure below shows a schematic diagram of the relative locations of some hybridization probes and PCR primer sets specific to the C. botulinum Type F Toxin gene. Information about each probe or primer set is given below. This list is not intended to be comprehensive, but to serve as a starting point.
The sequence of the C. botulinum Type F toxin gene can be found at GenBank accession M92906.
Hybridization Probes
| Probe # | Sequence (5'-3') | Reference |
| CBT01 | agacttgaatctgaatataatatcaata |
PCR Primers
| Primer Pair # | Primer A (5'-3') | Primer B(5'-3') | Reference |
| gcttcattaaagaacggaagcagtgct | gtggcgcctttgtaccttttctagg |
References
1. Campbell, K., M. Collins, and A. East. 1993. Gene Probes for Identification of the Botulinal Neurotoxin Gene and Specific Identification of Neurotoxin Types B, E, and F. J. Clin. Microbiol. 31; 2255-2262.
2. Ferreira, J., M. Hamdy, S. McCay, M. Hemphill, N. Kirma, and B. Baumstark. 1994. Detection of Clostridium botulinum Type F Using the Polymerase Chain Reaction. Mol. and Cell. Probes 8; 365-373.
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