PROBES AND PRIMER SEQUENCES SPECIFIC TO Clostridium difficile TYPE A TOXIN
The figure below shows a schematic diagram of the relative locations of some hybridization probes and PCR primer sets specific to the C. difficile Type A Toxin gene. Information about each probe or primer set is given below. This list is not intended to be comprehensive, but to serve as a starting point.
The sequence of the C. difficile Type A toxin gene can be found at GenBank accession M30307.
Hybridization Probes
| Probe # | Sequence (5'-3') | Reference |
| CDT01 | actggagcagttccaggtttaagatc |
PCR Primers
| Primer Pair # | Primer A (5'-3') | Primer B (5'-3') | Reference |
| gcatgataaggcaacttcagtgg | gagtaagttcctcctgctccatcaa | ||
| ggaaatttagctgcagcatctgac | tctagcaaattcgcttgtgttgaa |
References
1. Tang, Y., P. Gumerlock, J. Weiss, and J. Silva. 1994. Specific Detection of Clostridium difficile toxin A Gene Sequences in Clinical Isolates. Mol. and Cell. Probes 8; 463-467.
2. McMillan, D., L. Muldrow, and S. Laggette. 1992. Simultaneous Detection of Toxin A and Toxin B Genetic Determinants of Clostridium difficile Using the Multiplex Polymerase Chain Reaction. Can. J. Microbiol.38; 81-83.
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