PROBES AND PRIMER SEQUENCES SPECIFIC TO Clostridium difficile TYPE B TOXIN
The figure below shows a schematic diagram of the relative locations of some hybridization probes and PCR primer sets specific to the C. difficile Type B Toxin gene. Information about each probe or primer set is given below. This list is not intended to be comprehensive, but to serve as a starting point.
The sequence of the C. difficile Type B toxin gene can be found at GenBank accession X53138.
Hybridization Probes
| Probe # | Sequence (5'-3') | Reference |
| CDT03 | gaaattgcatattattataatcctggagatggt | |
| CDT12 | gtggagtgttacaaacaggtg |
PCR Primers
| Primer Pair # | Primer A (5'-3') | Primer B (5'-3') | Reference |
| aggtgaactactgtgcattc | gaaaaaatggctaatgt | ||
| ggtgatatggaggcatcaccactag | tccaggataagtctcctctacgttg |
References
1. Green, G., B. Riot, and H. Monteil. 1992. Identification of Toxigenic Clostridium difficile Strains Using a Toxin B Gene-Specific Oligonucleotide Probe. Mol. and Cell. Probes 6; 101-105.
2. Gumerlock, P., Y. Tang, J. Weiss, and J. Silva. 1993. Specific Detection of Toxigenic Strains of Clostridium difficile in Stool Specimens. J. Clinical Microb. 31; 507-511.
3. McMillin, D., L. Muldrow, S. Leggette, Y. Abdulahi, and U. Ekanemesang. 1991. Molecular Screening of Clostridium difficile Toxins A and B Genetic Determinants and Identification of Mutant Strains. FEMS Microbiol. Letts. 78; 75-80.
4. McMillan, D., L. Muldrow, and S. Laggette. 1992. Simultaneous Detection of Toxin A and Toxin B Genetic Determinants of Clostridium difficile Using the Multiplex Polymerase Chain Reaction. Can. J. Microbiol.38; 81-83.
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